As a promising material for biomedical application, bio-nanocomposites have attracted great attention due to their excellent mechanical properties, low density, and enhanced chemical inertness. Understanding tribological properties of bio-nanocomposites, namely adhesive force, frictional force, and wear (scratch) resistance, is one of the critical issue in designing and developing biomedical applications such as surgery tools and artificial joints. However, tribological issues of biomedical bio-nanocomposites have not been properly studied. We have worked to develop micro/nanocrystalline diamond coated implant materials having reduced friction which is suitable for hemiarthroplasty. Additionally, the crucial design factors of nanohoneycomb composites for reducing the capability of bacterial adhesion have been studied. We demonstrate mechanically robust, tribologically suitable, and biocompatible nanocomposites using metals, ceramics, and various carbon materials including graphene, graphene oxide, and diamond-like carbon (DLC) in conjunction with the self-assembly techniques for biomedical applications. This research will also explore how bio-nanocomposites can improve and optimize the lifetime of structures and part quality for biomedical devices.